MolCellEng_Notes9 - A.i....

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
A.i. SNP Genotyping by MALDI-TOF MS and SBE (single base extension) A.i.1. Have SNPs at diff locations A.i.2. Must already know where SNPs are and what their  genotypes are A.i.3. Not true discovery of SNPs, it is rather a Screening of  known SNPs for large number of samples to guess frequency of  SNP in population or to verify epidemic changes in genotype A.i.4. Design primer that sits right next to each SNP and primers  get extended at SNP position A.i.5. extension A.i.6. After extension reaction, will have unextended primer  (always add excess, so have some left over) and extension products  – then, purify sample (salt will interfere with mass analysis) A.i.7. Read genotype by looking at peaks; primers will have peaks A.i.8. Limitations A.i.8.a. Unwanted measurement of unextended primers with  the extension products   limits the scope of multiplexing A.i.8.a.i. If have 2 SNPs looking at, no issue b/c will have 6  peaks maximum – not too complicated spectrum A.i.8.a.ii. If have 20 SNPs, have max of 3 peaks for each SNP  – 60 peaks in spectrum, makes spectrum  complicated as seen in this slide’s picture.  A.i.8.b. Really don't need primer peaks, they bring down the  intensity of other extension products and lowers the 
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/06/2012 for the course BIO 436 taught by Professor Kim during the Spring '12 term at Rutgers.

Page1 / 3

MolCellEng_Notes9 - A.i....

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online