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lecture10 - Life Science 15: Concepts and Issues Lecture...

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Life Science 15: Concepts and Issues Lecture 10: Modern genetics and biotechnology: DNA fingerprinting 2/9/12 I. DNA fingerprinting overview II. Individual differences in DNA- VNTRs III. RFLP analysis and DNA fingerprinting IV. Difficulties and controversies Why are we interested in DNA fingerprinting? - real fingerprinting not left behind in many crime scenes - they are essentially unique DNA fingerprinting: - Uses: o Solve unsolvable crimes o Resolve paternity issue o Can prove innocence of people unjustly convicted o Immigration DNA is a long sequence of ‘letters’ that carry the information of how to build molecules for the body. A A G T C C A G G G G C T ________________________ T T C A G G T C C C C G A Four ‘letters’: A,T,C,G How much spelling difference is there between individuals? - almost none- 99.9% identical - different enough, though: 3 million different base pairs in that .1% On average, individuals differ in their DNA sequence at 0.1% of their bases: 3 million differences out of 3 billion base pairs. The fundamental question: We have DNA from several samples. How can we tell if the
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This note was uploaded on 04/10/2012 for the course LS 15 taught by Professor Phelan during the Spring '08 term at UCLA.

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lecture10 - Life Science 15: Concepts and Issues Lecture...

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