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Unformatted text preview: F. Add buffer, water, and DPIP to cuvettes. G. Add 3 drops of chloroplasts (boiled and unboiled) to the appropriate tubes, after this use Parafilm over the tops of the tubes and mix the contents. H. Wipe the tubes clean. I. Place calibration tube into the sample holder, align it, close the lid and adjust the spectrophotometer until it reads 100% transmittance. J. Repeat with the 3 other cuvettes. K. Read the cuvettes by placing them into the sample holder. L. Record results and read the cuvettes after three more 5 minute intervals. M. Draw a data chart....
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- Spring '08
- Chloroplast, Laboratory glassware, Erlenmeyer flask, large Erlenmeyer flask, Ian Lay, Number your cuvettes