Bacterial Transformation Lab Report .pdf - Bacterial...

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Bacterial Transformation Lab ReportBIO 181 Thursday 10:30AM-1:15PM Taylor Ballantyne October 13, 2016
AbstractBacteria is defined as microscopic organisms that are typically found everywhere, usually being one-celled. For this lab, bacteria will be transformed with DNA so that it expresses different genetic information. The purpose of this lab is to explore the effects of bacterial transformation using E. Coli. Plasmid DNA will be inserted into the E. Coli bacteria and procedures such as heat shock and incubation will be performed. As a group, our belief is that the bacterium with the arabinose sugar and plasmid injected into it, will produce bacteria colonies that will glow. We observed that the (-)pGLO LB plate presented lots of growth, (-)pGLO Amp, LB plate had no growth, (+)pGLO Amp, LB plate had some growth, (+)pGLO Ara, LB plate had some growth, and lastly, (+)pGLO Amp, Ara, LB plate had lots of growth as well. IntroductionBacteria transformation is defined as the process by which bacterial cells take up naked DNA molecules. If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. For the bacterial transformation lab, we learned about the process of moving genes from one organism to another with the help of a plasmid. The method that we used was bacterial transformation. As a group we used a procedure to transform bacteria with a plasmid containing a gene that codes for the green fluorescent protein. If the procedure deems successful, the 1
bacteria will express the fluorescent protein under an ultraviolet light. The bacteria we used was E. coli, arabinose sugar, and glowing fluorescent proteins. By having an antibiotic resistant gene in the plasmid, it allows the plasmid to be easily detectable when the cells are grown on selective media, which also provide these bacteria with pressure to keep the plasmid. By adding arabinose

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Term
Spring
Professor
Harme
Tags
Bacteria, DNA, Escherichia coli, pGLO LB plate

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