termsConcepts4 - Key terms and concepts in unit 4(lectures...

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Key terms and concepts in unit 4 (lectures 24-31) Recombination, cancer (Lecture 24) recombination: any new combination of genes recombination can involve gene and chromosome transfers brought about by prokaryotes conjugation (introduced in a later lecture) eukaryotes meiosis, independent assortment zygosis (fusion of gametes such as sperm and egg) molecular recombination involves breaking and rejoining of DNA by crossing over at prophase I of meiosis by transposition transposition involves stretches of DNA called transposons catalyzed by enzymes called transposases carcinoma sarcoma oncogenes proto-oncogenes (encode positive regulators of cell cycle) detection of oncogenes by transfection assay tumor suppressor genes encode negative regulators of cell cycle oncogenes are dominant alleles mutant tumor suppressor genes are recessive alleles DNA technology (lectures 25-26) Restriction endonucleases What they do, their uses in DNA technology, specificity Role in nature Gel electrophoresis How it works, for what is it used RFLPs and DNA fingerprinting DNA cloning "sticky ends" recombinant DNA cloning vector purpose of vector, properties that make a good vector
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This note was uploaded on 04/07/2008 for the course PRE MED 101 taught by Professor Bio2,chem24,bio100 during the Winter '08 term at Marquette.

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termsConcepts4 - Key terms and concepts in unit 4(lectures...

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