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Unformatted text preview: n spectrum for the diluted
sample will have absorption values = 1/3
the values shown in the above spectrum.
2. The absorptivity coefficient, Ε, increases
from λ 400 to λ 450, in the absorption
spectrum for the diluted sample. Correct Not Correct 4 points Page 4/10 Name: ___________________________________page 5/10
C. What is the slope of the above calibration graph line (λ=600 nm) in units of absorbance/M? slope = _______ abs/M
2 points D. You produce a second calibration graph for Trans Ion at λ=450 nm. You use the same
samples, sample holder, and spectrophotometer. Will the slope of the resulting calibration
graph line be more, less, or the same as the above calibration line at λ=600 nm?
Indicate (circle) if the slope of calibration graph line at λ=450 will be
more than less than the same as the slope at λ=600
3 points E. Use the Beer-Lambert Law and the above calibration graph to determine the unknown concentration
(M) of a solution of Trans Ion that has an absorbance of 0.30 at λ = 600 nm. Show all calculations for
credit. You will not get credit for numerical answers alone. Trans Ion M = ______...
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This note was uploaded on 05/21/2012 for the course CHEM 125 taught by Professor Goddfried during the Fall '06 term at University of Michigan.
- Fall '06
- Periodic Table