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Unformatted text preview: 1. The following prokaryotic DNA sequence encodes an mRNA. a. Circle the promoter consensus sequences found on the coding strand of DNA 2 pts b. Write out the mRNA sequence encoded by this gene (indicate the 5 and 3 ends). 4 pts c. Translate that mRNA sequence into an amino acid sequence using the provided genetic code (indicate the N and C termini of the polypeptide). 4 pts 5 AACATTGACACCGTAACTGTACGCTCATATATTCGATCGGCTTAGATGCCT GCTTACCGGGAGTAAGCCATCGA 3 3 TTGTAACTGTGGCATTGACATGCGAGTATATAAGCTAGCCGAATCTACGGACGAATGGCCCTCATTCGGTAGCT 5 mRNA sequence: amino acid sequence: 2. The following table shows part of the sequence of a gene, with both DNA strands, the mRNA strand and the amino acid sequence indicated. Complete the table, including all of the nucleotides and amino acids. Label the 5 and 5 ends of each nucleotide strand. 2.5 pts 1 2 3 4 5 6 7 8 9 10 11 12 DNA double helix-- C G T C A T A T C AG G T T G A G A MRNA GC A U G G A Amino acids Trp Ser 3. The normal sequence of a particular protein is given here, along with several mutant versions of it. For each mutant, explain what mutation occurred in the coding sequence of the gene. Assume only a single mutational event explains each altered amino acid sequence. 4 pts () = multiple unspecified amino acids * On the final, you would only be asked about one mutant. Normal: Met-Gly-Glu-Thr-Lys-Val-Val-()-Pro Mutant 1: Met-Gly Mutant 2: Met-Gly-Glu-Asp Mutant 3: Met-Gly-Arg-Leu-Lys Mutant 4: Met-Arg-Glu-Thr-Lys-Val-Val-()-Pro 4. In the replication bubble below, label all ends of newly made DNA (in bold) with either 5 or 3 (as shown in one example below): 2 pts...
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