Lecture 19

# Why couldnt a membrane atpase drive this movement 4

This preview shows page 1. Sign up to view the full content.

This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: the IMM is highly impermeable, not leaky. An ATP/ADP exchanger in the IMM accomplishes this, driven by the charge gradient across the IMM. Why couldn’t a membrane ATPase drive this movement? (4) ATP synthase disco, some students will do anything for extra credit: DVD 14.4 <so, the ion gradient across the IMM drives ATP synthesis as well as transport. We can calculate the amount of energy stored in that gradient and assess whether it is adequate to perform specific kinds of work, just as we did for the Na+ gradient and the Na+/glucose symporter:> (C) Calculating the amount of energy in the proton gradient. ÄG = F(Äp) Äp is the “protonmotive force,”and Äp = ø ￿ 59mV(ÄpH). ÄpH = pHcytoplasm (IMS) ￿ pH(matrix) ø = the Vm of the inner mitochondrial membrane. Typically, ø is a large positive number (e.g., +100 to +200 mV) because the matrix is negatively charged with respect to the intermembrane space due to the removal of protons from the matrix (during electron transport). For example, if ø = + 200 mV, pH intermembrane space = 7, and pH matrix = 8, then ÄpH = ￿1 and Äp = 200 mV ￿ (￿59 mV) = +259 mV. And thus ÄG = F(Äp) = +25.9 kJ/mole. Because ÄG > 0, the spontaneous movement of protons will be OUT of cytoplasm and into matrix. So under these conditions, one mole of H+ will yield 25.9 kJ. H e r e i s a g o o d r e p r e s e n t a t i o n o f m u c h o f w h a t w e ’ v e t a lk e d a b o u t h e r e : T C A c y c le , e l e c t r o n t r a n s p o r t , A T P s y n t h e s i s , a n d t r a n s p o r t o f m e t a b o l i t e s a c r o s s th e m it o c h o n d r ia l m e m b r a n e . I t is F ig . 5 . 3 0 f r o m K a r p ’s C e ll & M o le c u la r B io lo g y ....
View Full Document

## This note was uploaded on 06/12/2012 for the course BIOLOGY 231 taught by Professor Petethollenbeck during the Fall '10 term at Purdue.

Ask a homework question - tutors are online