Lambda Project .docx - Digestion of Linear Lambda DNA by...

This preview shows page 1 - 7 out of 13 pages.

Digestion of Linear Lambda DNA by Three Restriction Endonuclease Enzymes Bam HI , Eco RI and Hin DIII Using NEB Cutter Moksha Patel Madison and Dr. Martina Hausner BLG 888 March 4, 2020
1 Tables Table 1. Data representing fragment sizes generated by single digest of linear lambda DNA by restriction endonuclease Bam HI in order from longest to shortest. # Ends Length (bp) 1 Bam HI -Bam HI 16841 2 Bam HI -Bam HI 7233 3 Bam H I -(RightEnd) 6770 4 Bam HI -Bam HI 6527 5 Bam HI -Bam HI 5626 6 (LeftEnd)- Bam HI 5505 Number of Restriction Sites 5
2 Table 2. Data representing fragment sizes generated by single digest of linear lambda DNA by restriction endonuclease enzyme EcoRI in order from longest to shortest # Ends Length (bp) 1 ( LeftEnd)- Eco RI 21226 2 Eco RI -Eco RI 7421 3 Eco RI -Eco RI 5804 4 Eco RI -Eco RI 5643 5 Eco RI -Eco RI 4878 6 Eco RI - (RightEnd) 3530 Number of Restriction Sites 5
3 Table 3. Data representing fragment sizes for the digest of linear lambda DNA by restriction endonuclease enzyme Hin DIII from longest fragment size to shortest fragment size. # Ends Length (bp) 1 (LeftEnd)- Hin DIII 23130 2 Hin DIII - Hin DIII 9416 3 Hin DIII - Hin DIII 6682 4 Hin DIII - (RightEnd) 4361 5 Hin DIII - Hin DIII 2322 6 Hin DIII - Hin DIII 2027 7 Hin DIII - Hin DIII 564 Number of Restriction Sites 6
4 Table 4. Data representing fragment sizes for the digest of linear lambda DNA by restriction endonuclease enzymes Bam HI + Eco RI from longest fragment size to shortest fragment size # Ends Length (bp) 1 Bam HI -Eco RI 15721 2 (LeftEnd) - Bam HI 5505 3 Bam HI - Eco RI 4669 4 Bam HI - Eco RI 3775 5 Bam HI – Eco RI 3758 6 Eco RI - (RightEnd) 3530 7 Bam HI – Eco RI 3240 8 Eco RI - Bam HI 2752 9 Eco RI - Bam HI 2564 10 Eco RI - Bam HI 1868 11 Eco RI - Bam HI 1120 Number of Restriction Sites Bam HI 5 Eco RI -5
5 Table 5. Data representing fragment sizes for the digest of linear lambda DNA by restriction endonuclease enzymes Eco RI + Hin

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture