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Experiment 4: The Use of Western Blot in Detection of -galactosidase ProteinMoksha PatelMadison Dr, Hausner BLG 888Apr 7, 2020
1Overall ObjectiveThe objective of this experiment is to detect the presence of a protein of interest, particularly the -galactosidase, from one of the two strains of Escherichia coli, the EMG 26 K-12 lac-(i+z-y+) and the EMG 9 K-12 lac-(i-z+y+) strain, by using Western Blotting techniques and electrophoresis.
2ResultsTable 1. Distance travelled by protein markers of Tris-Glycine 4-20% during electrophoresisThe Protein marker of Tris-Glycine 4-20% produced 12 bands. The bands were used to calculate the relative distance travelled in an SDS-polyacrylamide gel electrophoresis. The distance travelled by each band was calculated using a ruler. The molecular weight of the protein was given, the log of the molecular weight was calculated along with the Rf values. Molecular Weight(kDa)Log MolecularWeightDistance Travelled(cm)Rf value2452.390.001802.260.20.0251352.130.50.0641002.001.00.13751.881.50.19631.802.10.27481.683.00.38351.544.00.51251.405.30.68201.305.90.76171.237.00.90111.047.81.0