Chromatography

Chromatography - Chromatography Chemistry 1 Lab Course 2207...

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Chromatography September 18, 2007 Chemistry 1 Lab Course 2207 Hsiang-Ting Ko Kelsey Lynch
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Purpose: The purpose of this experiment is to learn how to separate compounds individually by way of chromatography. It also is meant to show how the stationary and mobile phases affect the movement of molecules and how changing the various liquids in the mobile phase affect the speed of the compound moving down the column. In this experiment one learns how to separate the acetone spinach extract and the colors of the dye in M&M’s. The purpose of the paper chromatography experiment is also to see which dyes are pure in M& M’s. Procedure: Not to be included (see notebook). Data/Observations (tables): For column Chromatography of Leaf Pigments: Speed of solvent flow (drops/second) and observed color of eluted solvent Test Tube Number Solv-ent Used Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6 hexane 13 (clear) 14 (light green) 15 (darker green) 15 (darkest green) 13 (darker green) 10 (light green) acetone 15 (light green) 15 (very faint green) 16 (clear- hint of green) 13 (clear) 11 (clear) (not enough solvent left) 70% isopropyl alcohol 5 (clear w/ faint touch green) 5 (yellow tint) 3 (clear) 4 (more greenish) Not enough Not enough
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Important observation: The sodium bicarbonate is tinted slightly green after hexane drip and becomes greener after the acetone solvent is dripped out. When sodium bicarbonate is added the band becomes darker green. The band makes its way down the tube farther and farther as each solvent drip is allowed to occur. Also, the cotton is spotted green during the hexane solvent drip. The importance and meaning of these observations is explained in the results. Color Retent-ion Factor * observations of the chromatogram show that green and brown colors contain yellow dye #5 and are not pure colors Standard yellow Green (separates into yellow
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This note was uploaded on 04/07/2008 for the course CHEM 001 taught by Professor Babikov during the Spring '08 term at Marquette.

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Chromatography - Chromatography Chemistry 1 Lab Course 2207...

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