Unformatted text preview: ADP~P bond(!) Note the rotor (base) and stator (head) and rotation F1 F0 Hypothesis: ADP + Pi
ATP + H2O occurs on head;
H+ flow turns rotor; rotor/rotation stimulates ATP release rotor
rotation Figure 1 Observation system for the c subunit rotation in F0F1. Y Sambongi et al. Science 1999;286:1722-1724 Movie: http://www.sciencemag.org/site/feature/data/1045705a.mov Movie of the ATPase model -- Mechanical part of respiration.
http://multimedia.mcb.harvard.edu/anim_mitochondria.html Model for H+-induced rotation from the textbook: What is the P:O ratio ?
ATP formed: 1/2 O2 (2 e-) taken up
For TCA cycle, early estimates: per NADH, 3; per
succinate (FAD), 2
Best data: per NADH, 2.5; per succinate (FAD), 1.5
Book: 10 H+ per NADH, 4 H+ per ATP = 2.5
(Originally depended on an accurate measure of H+
flow, correcting for leaks across the membrane; now
related to the Fo structure: 10 H+/NADH,
12 H+/Fo rotation, 3 ATP/Fo rotation)
Recent evidence: H+/ATP varies from 12/3 (4) in
animals to 15/3 (5) in microbes, depending on the
number of Fo subunits in the Fo ring in the
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This note was uploaded on 08/30/2012 for the course BIS 105 taught by Professor Kennethhilt during the Spring '09 term at UC Davis.
- Spring '09