ATPsynthesis

Ao ao ao ao add atp m embrane vesicle containing atp

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Unformatted text preview: sis powers H+ flow. AO AO AO AO Add ATP M embrane vesicle containing ATP synthase AO+ + (outside) AO AO+ H+ + • S o l u t ion of acrydine orange (AO) AO (membrane-permeable fluorescent dye) AO+ • Add ATP, vesicle interior becomes acidic ATP ADP + Pi • AO AO+, trapped inside vesicle • High concentration of AO quenches fluorescence • 2. H+ flow in artificial vesicle powers ATP synthesis. Light H+ Bacteriorhodopsin H+ H+ H+ Add ADP H+ H+ H+ H+ ADP + Pi ATP H+ 3. Uncouplers — compounds that permeabilize the mitochondrial inner membrane to H+ inhibit ATP synthesis (and allow rapid oxidation of substrates like pyruvate) FCCP FCCP FCCP-H+ H+ H+ H+ H+ H+ H+ (FCCP: carbonylcyanide p-trifluoromethoxyphenylhydrazone) 4. Reversible oxygen exchange 18 O from H218O incorporated into HPO42- in the presence of the ATP synthase Without unidirectional H+ flow, ADP + Pi ATP + H2O occurs reversibly, implying that the application of energy is not at the formation of the...
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