sp04-3 - FINAL EXAM, Biochemistry 102/2004 I. 1 (50 points)...

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1 FINAL EXAM, Biochemistry 102/2004 I. (50 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the left to the product on the right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, P i , NAD + , NADH, etc.), and any required prosthetic groups (e.g., TPP, FAD, biotin, etc.). A. acetyl CoA (cytosol) O CH 3 CH 2 CH 2 C–SACP
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2 B. Pyruvate in matrix (gluconeogenesis in liver) Phosphoenolpyruvate _____________________________________________________________________(draw)_______________ C. 3-Phosphoglycerate (gluconeogenesis in liver) (draw) F r u c t o s e - 6 - P h o s p h a t e _______________________________________________________________________(draw)_____________ D. Glucose G l y c o g e n (indicate the point on the structure of the polymer where the entering glucose attaches)
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3 II. (10 points) Answer one of the following two problems. Please cross out the one that you don't wish to have graded. A. Outline the steps starting with an increase in adenylate cyclase activity in the liver on the left and ending with the relevant changes in the activities of glycogenolysis and gluconeogenesis in the liver on the right. Use arrows ( ↑ or ) to show the effect on messenger molecule concentration, enzyme activity, and pathway activity at each step, as in the lecture handout “Responses in liver cells when blood glucose levels are low.” B. Show the complete mechanism of the Rubisco catalyzed reaction of ribulose 1,5 bisphosphate and CO 2 to yield 3 phosphoglycerate, at the level of the lecture handout.
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4 III. (20 points) Diagram the overview of proton and electron circuits in chloroplast thylakoids starting with H 2 O and ending with the formation of O 2 , NADPH, and ATP (the diagram on the lecture handout will receive full credit). Label each entity (photosystem, protein, etc.), indicate stroma, lumen, and thylakoid membrane, and show the flow of protons, electrons, and photons.
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5 IV. (20 points) In the space below please draw all of the enzymatic reactions needed to convert the amino nitrogen of alanine to a nitrogen of urea. Assume that your starting materials are alanine, α -ketoglutarate, aspartate, arginine , and bicarbonate . Draw the correct structure for each intermediate molecule; with the sole exception of the urea cycle intermediates, where you can either give the correct structure or the correct name. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD + , FAD, NADH, etc.), any required cofactors (e.g., biotin, TPP, etc.)
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6 V. (200 points) Multiple Choice . Choose only one answer for each question and enter it on the accompanying computer sheet. Please show all work on your exam for problems which involve calculations. 1.
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This test prep was uploaded on 04/07/2008 for the course BIBC 102 taught by Professor Price during the Spring '02 term at UCSD.

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sp04-3 - FINAL EXAM, Biochemistry 102/2004 I. 1 (50 points)...

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