sp05-2 - 1 FINAL EXAM Biochemistry 102/2005 Name I(25...

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Unformatted text preview: 1 FINAL EXAM, Biochemistry 102/2005 Name__________________________________ I. (25 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the upper left to the product on the lower right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD + , FAD, NADH, etc.), any required cofactors (e.g., biotin, TPP, etc.), and indicate subcellular location ( cytosol or matrix ). O A. CH 3 (CH 2 ) 14 C-O ( Cytosol ) O O CH 3 (CH 2 ) 12 C SCoA + CH 3 C SCoA (matrix) (matrix) B. Please indicate which one of the above enzymatic reactions is under allosteric regulation , the identity of the allosteric activators and/or inhibitors of this enzyme, and the physiological role of this regulation in a cell. 2 II. ( 25 points ) Fill in all of the enzyme catalyzed reactions which convert the substrate on the left to the product on the right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, P i , NAD + , NADH, etc.), and any required prosthetic groups (e.g., TPP, FAD, biotin, etc.). (A) Acetyl CoA (cytosol) O CH 3 CH 2 CH 2 C–SACP (B) Briefly describe the function of the acyl carrier protein in the reactions catalyzed by fatty acid synthase and name the coenzyme from which the prosthetic group of acyl carrier protein is derived. 3 III . ( 25 points ) Fill in all enzyme catalyzed reactions needed to convert the substrate on the left to the product on the right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, P i , NAD + , NADH, etc.), and any required prosthetic groups (e.g., TPP, FAD, biotin, etc.). (A) Pyruvate (gluconeogenesis in liver) ( Indicate which enzymatic reactions are different from the reactions used in glycolysis, and indicate which steps occur in the matrix and which occur in the cytosol.) Glyceraldehyde-3-phosphate (draw) ------------------------------------------------------------------------------------------------------ (B) Glucose (glycogen synthesis in the liver) (Show the point on the glycogen polymer to which the entering glucose attaches.) (Glucose) n ↓ 4 (Glucose) n+1 IV.(25 points) Draw out all of the enzymatic reactions needed to convert the 15 N labeled amino nitrogens of glutamate and aspartate to urea. Assume that your starting materials are glutamate , aspartate , arginine , and bicarbonate . Include the names or abbreviations of any other reactants and products (eg., ATP, ADP, P i , NAD + , etc.) and any required cofactors (eg., biotin, TPP, etc.) ( Note :you should draw the correct structure for each intermediate molecule, but you don’t need to draw the structure of the enzyme bound intermediates.) 5 V . ( 30 points ) Fill in all enzyme catalyzed reactions needed to convert the substrate on the left to the product...
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This test prep was uploaded on 04/07/2008 for the course BIBC 102 taught by Professor Price during the Spring '02 term at UCSD.

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sp05-2 - 1 FINAL EXAM Biochemistry 102/2005 Name I(25...

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