sp06 final exam

sp06 final exam - FINAL EXAM Biochemistry 102/2006 I 1...

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1 FINAL EXAM, Biochemistry 102/2006 Name__________________________________ I. (25 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the upper left to the product on the lower right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD + , FAD, NADH, etc.), any required cofactors (e.g., biotin, TPP, etc.), and indicate subcellular location ( cytosol or matrix ). A. CH 3 (CH 2 ) 14 CO 2 ( Cytosol ) CH 3 (CH 2 ) 12 C(O) SCoA + CH 3 C(O) SCoA (matrix) (matrix) B. Please indicate which one of the above enzymatic reactions is under allosteric regulation , the identity of the allosteric activators and/or inhibitors of this enzyme, and explain the physiological role of this regulation in a cell.
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2 II. ( 25 points ) Fill in all of the enzyme catalyzed reactions which convert the substrate on the left to the product on the right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, P i , NAD + , NADH, etc.), and any required prosthetic groups (e.g., TPP, FAD, biotin, etc.). (A) Acetyl CoA (cytosol) CH 3 CH 2 CH 2 C(O)–SACP (B) Briefly describe the function of the acyl carrier protein in the reactions catalyzed by fatty acid synthase and name the coenzyme from which the prosthetic group of acyl carrier protein is derived.
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3 III . ( 30 points ) Fill in all enzyme catalyzed reactions needed to convert the substrate on the left to the product on the right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, P i , NAD + , NADH, etc.), and any required prosthetic groups (e.g., TPP, FAD, biotin, etc.). (A) Pyruvate in matrix (gluconeogenesis) (draw) Phosphoenolpyruvate in cytosol (draw) ------------------------------------------------------------------------------------------------------ (B) Glucose in the liver cell (Show the point on the glycogen polymer to which the entering glucose attaches.) Glycogen (show the point on the glycogen polymer to which the entering glucose attaches) -------------------------------------------------------------------------------------------------------------------------- (C) 3-phosphoglycerate Fructose-6-phosphate
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4 IV. (25 points) Draw out all of the enzymatic reactions needed to convert the 15 N labeled amino nitrogens of glutamate and aspartate to urea. Assume that your starting materials are glutamate , aspartate , arginine , and bicarbonate . Include the names or abbreviations of any other reactants and products (eg., ATP, ADP, P i , NAD + , etc.) and any required cofactors (eg., biotin, TPP, etc.) ( Note :you should draw the correct structure for each intermediate molecule, but you don’t need to draw the structure of the enzyme bound intermediates.) (If a structure is incorrectly drawn, you will receive half credit for the correct name of this structure).
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sp06 final exam - FINAL EXAM Biochemistry 102/2006 I 1...

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