Lab Report – Lab 3 Addendum – Cell Anatomy and Physioligy
Mark R. Graham
1455 Betty Court, Orange Park FL 32073
BSC2085C - Anatomy and Physiology I - 333738
Fall Term 2010
Larry Chad Winter
Submitted - 9/18/2010
Cell Transport Mechanisms and Permeability
Simulating Dialysis (Simple Diffusion)
Chart 1 – Dialysis Results
Which solute(s) were able to diffuse into the right beaker from the left? Na+/Cl- , Urea, Glucose
Which solute(s) did not diffuse? Albumin
If the solution in the left beaker contained both urea and albumin, which membrane(s) could you
choose to selectively remove the urea from the solution in the left beaker?
100 MCWO and 200
Assume that the solution in the left beaker contained NaCl in addition to the urea and albumin.
How could you set up an
experiment so that you removed the urea, but left the NaCl concentration unchanged? Put a
solution in the right beaker with no urea and the same concentration of
NaCl as the left beaker
while using a 100 or 200 MCWO membrane between the two beakers.
A c t i v i t y 2: Simulating Facilitated Diffusion
Chart 2 – Facilitated Diffusion Results
|Glucose Concentration (mM)
|Number of glucose carrier proteins
What happened to the rate of facilitated diffusion as the number of protein carriers increased?
Explain your answer.
The rate of facilitated diffusion of glucose molecules increased and the time needed to reach
equilibrium decreased with the successive increases of glucose carrier proteins placed in the
membranes used in the experiments recorded above. This means that glucose carrier proteins
assist in speeding up the diffusion of glucose across a permeable membrane.
What do you think would happen to the transport rate if you put the same concentration of
glucose into both beakers instead
of deionized water in the right beaker? The transport rate would be equal in both directions
meaning the solutions would be at equilibrium.
Should NaCl have an effect on glucose diffusion? Explain your answer. Use the simulation to see
if it does.
The sodium and chloride ions should have no impact on the diffusion of glucose through the
membrane. The simulation proves this to be true no matter how many glucose carriers are in the
membrane or how high the concentration of NaCl is in either beaker. The membrane is loaded
with proteins that assist in the diffusion of only glucose.
A c t i v i t y 3 :
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