Wk 2 Lab answers - REVIEW SHEET Lab Report – Lab 3...

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REVIEW SHEET Lab Report – Lab 3 Addendum – Cell Anatomy and Physioligy PhysioEx Worksheet Mark R. Graham 1455 Betty Court, Orange Park FL 32073 BSC2085C - Anatomy and Physiology I - 333738 Fall Term 2010 Larry Chad Winter lwinter@fscj.edu Submitted - 9/18/2010 NAME Mark Graham Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Chart 1 – Dialysis Results |Membrane (MCWO) | |Solute |20 |50 |100 |200 | |NaCl |0.0000 |0.0150 |0.0150 |0.0150 | |Urea |0.0000 |0.0000 |0.0094 |0.0094 | |Albumin |0.0000 |0.0000 |0.0000 |
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0.0000 | |Glucose |0.0000 |0.0000 |0.0000 | 0.0040 | Which solute(s) were able to diffuse into the right beaker from the left? Na+/Cl- , Urea, Glucose Which solute(s) did not diffuse? Albumin If the solution in the left beaker contained both urea and albumin, which membrane(s) could you choose to selectively remove the urea from the solution in the left beaker? 100 MCWO and 200 MCWO Assume that the solution in the left beaker contained NaCl in addition to the urea and albumin. How could you set up an experiment so that you removed the urea, but left the NaCl concentration unchanged? Put a solution in the right beaker with no urea and the same concentration of NaCl as the left beaker while using a 100 or 200 MCWO membrane between the two beakers. A c t i v i t y 2: Simulating Facilitated Diffusion Chart 2 – Facilitated Diffusion Results |Glucose Concentration (mM) |Number of glucose carrier proteins | | |500 |700 |900 | |2.00 |0.0150 |0.0150 |0.0150 | |8.00 |0.0025 |0.0034 |0.0042 | What happened to the rate of facilitated diffusion as the number of protein carriers increased? Explain your answer.
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The rate of facilitated diffusion of glucose molecules increased and the time needed to reach equilibrium decreased with the successive increases of glucose carrier proteins placed in the membranes used in the experiments recorded above. This means that glucose carrier proteins assist in speeding up the diffusion of glucose across a permeable membrane. What do you think would happen to the transport rate if you put the same concentration of glucose into both beakers instead of deionized water in the right beaker? The transport rate would be equal in both directions meaning the solutions would be at equilibrium. Should NaCl have an effect on glucose diffusion? Explain your answer. Use the simulation to see if it does. The sodium and chloride ions should have no impact on the diffusion of glucose through the membrane. The simulation proves this to be true no matter how many glucose carriers are in the membrane or how high the concentration of NaCl is in either beaker. The membrane is loaded with proteins that assist in the diffusion of only glucose. A c t i v i t y 3 :
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