Hemoglobin DeterminationBy:Vaughn Ogrydziak, Irish Rives, Paul Rizk, Ethan Stewart, Sarah Woodside
Abstract 1•Two ordinary green light-emitting diodes–light emitter and detector coupled with simple voltmeter –cost-effective prototype of a photometric hemoglobinometer.•Hemoglobin (Hb)–Ferrous oxygen transport metalloprotien in red blood cells•Hemoglobinometer–a digital photometer for measurement of blood hemoglobin concentration by its optical density.•Quantitative assays–measure the amount of a substance in a sample.
Abstract 2•Three methods used –Drabkin reagent–Lauryl sulfate –Dithionite methods•The utility of developed device for real world analysis has been validated through experimentation.•Results fully compatible with those obtained using clinical methods and analysis.
Analyses performed outside specialized laboratories•Biomedical tests at home•Food quality control•Environmental screening and monitoring
Light Emitting Diodes (LEDs)•Simplest and cheapest optoelectronic components•Mass-produced in semiconductor technology•Robust•Low power•Intensive light emitters•Narrow emission spectra covering broad spectral range from UV to NIR•Widely applied in photometric systems as effective light sources
Internal photoelectric effect•Opposite to electroluminescence phenomenon•Allows the use of LEDs as light detectors Einstein discovered the photoelectric effect•Therefore, the paring of two LEDs play the role of a light emitter and detector which leads to construction of a complete photometric device useful in analytical chemistry.
Paired Emitter Detector Diode (PEDD)•Photometer that generates reproducible, hundred-millivolts analytical signals•ex.–A complete PEDD-based photometer made of two red LEDs couple with an ordinary voltmeter, developed for education needs and useful as an efficient analytical tool for determination of blue dyes, pH, and pH-indicators as well as for kinetic assays of enzymes activity, has been demonstrated only recently
To Sum It Up•A very simple PEDD-based device dedicated for photometric determination of hemoglobin(Hb) is presented.