Prac2 report.docx - PHOTOSYNTHESIS INTRODUCTION The present...

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PHOTOSYNTHESISINTRODUCTION:The present of carbon dioxide, water and light energy from the sun allow the plants toundergo photosynthesis to produce glucose and oxygen. Photosynthesis takes place in the chloroplasts. Moreover, photosynthesis is important to plant because the production of sugar is acts as a fuel for the plants activities (eNotes 2015). Photosynthesis divides into two stages which are light and dark reactions and each undergoes multiple steps. In the light reaction, electrons are used to produce NADPH and ATP with the help of photosystem I (PSI) and II (PSII) by maximizing the collection of light energy. Light must be absorbed twice to remove the electrons from water and pass through PSII and PSI before ending up in NADPH. Hence, this process known as photophosphorylation because it uses light energy to make ATP from ADP (Douglas & Jean 2016). In this experiment, chloroplast with different conditions will be tested out with DCPIP which acts as an electron acceptor and LIGHT Tube 3 is expected to have the lowest absorbance value among all other tubes. As more electrons produced, the colour of the DCPIP decreases rapidly. Thus, this shows an increase in rate of photosynthetic electron transport.
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