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BIO 181R notes H and G, Recombinant DNA Techniques

BIO 181R notes H and G, Recombinant DNA Techniques -...

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Recombinant DNA Isozymes, different genes for the same reaction cGMP phosphodiesterase in the endothelial cells for males is a different enzyme than the cGMP phosphodiesterase in our eyes. Viagra is more specific for the endothelial cell form, but can cross react with the vision form. This is a common mechanism for having similar signaling pathways with different regulation in our tissues. Study Guide Know the definations. Definitions Recombinant DNA DNA molecule produced artifically and containing sequences from unrelated organisms. Genetic Engineering Use of techniques involving recombinant DNA technology to produce molecules and/or organisms with new properties. Biotechnology All inclusive term for several technologies including but not limited to recombinant DNA. Refers to the use of technology in applications for solving fundamental problems in biology. Genomics Study of genes expressed in cells. Proteonomics Study of proteins produced in cells. Overview an introduction to DNA technology simple techniques, leading to a revolution in biology simplicity of DNA (Watson Crick) exploited to understand life Nothing in biology astonished me until after 1966." Francis Crick Introduction to DNA Technology Study Guide
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Understand the signifance and function of the restriction endonucleases, including their use in modern biology. Restriction endonucleases restriction endonucleases -- cut double stranded DNA at specific sequences protect against viruses in bacteria Sequences may be palindromes: a sequence which is the same when read in either direction. "MOM" Example of Specificity of Restriction Endonucleases
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Study Guide Explain how gel electrophoresis separates nucleic acids or proteins. Gel Electrophoresis DNA fragments have a "-" charge
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a porous gel acts as a sieve, holding up big fragments fragments take up a dye that fluoresces under UV light Study Guide Describe the Southern technique. The Southern Technique labeled probe complementary to desired DNA sequence hybridization can show migration position of known DNA sequence Study Guide Describe the steps involved in cloning DNA, including what happens at each of the steps leading to a clone of bacteria with a copy of the desired gene. Recombinant DNA
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EcoR1 cuts on two different strands of DNA at lower temperature, the fragments can pair DNA ligase (joins Okazaki fragments) can generate recombinant DNA Introducing Recombinant DNA into Cells Plasmid Vectors
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small extrachromosomal DNA (2000-6000 bp) in a bacterial cell which can replicate independently but which cannot integrate into the host chromosome
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