Which of the following was a source of tension between English colonists and Native Americans?•The massacre of the Iroquois Confederacy changed Native American and colonial perceptions of one another. •Livestock belonging to Native Americans disrupted English agricultural practices. •Colonists viewed Native Americans as an impediment to their economic expansion. •The Second Anglo-Powhatan War significantly disrupted what had historically been peaceful relations between the two groups. CONCEPT English and Native American Conflict16 Many Native Americans were eager to trade with Europeans for advanced weaponry.