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Lab 7: Determination of Fe by UV-vis SpectroscopyI. Objective: To use UV-vis spectroscopy and a calibration curve to determine the absorbance of an unknown sample containing Fe and use Beers law to find Ɛ based on max absorbance.II. Material. 1,10-phenanthroline solution, standard iron(II) stock solution, hydroxylammonium chloride solution, sodium acetate solution, unknown solution, volumetric flasks, pipet, micro pipet, UV-vis Spectroscope, test tubesIII. Procedures:1)pipet in 5 volumetric flasks 5,10,15,20,25 mL of standard iron(II) solution in that order and label according to volume2)pour 50 mL of DI water in a sixth flask to serve as blank3)pipet 12mL of unknown solution in a seventh flask4)in order pipet into each flask a)1mL hydroxylammonium chlorideb)5mL 1,10-phenanthroline solutionc)8mL sodium acetate solution5)swirl flasks to mix solution then add to mark DI water. Place stopper in each and invert a few times to insure solution is homogeneous 6)wait 15 minutes for color to fully form