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bio18midterm2studyguidef07 - Bio 18 Fall 2007 Midterm 2...

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Bio 18 Fall 2007 Midterm 2 Study Guide Page 1 Bio 18 Exploring Biotechnology Fall 2007 Dr. Wong Midterm 2 Study Guide Standard Disclaimer : This study guide is intended to help you to focus your studying on major topics, concepts and skills. However , EVERYTHING covered in lecture, lab and relevant readings is fair game for an exam question. Lecture 10 Alu PCR Reading: Ch. 13 Sec. 13.3 1. Outline the steps involved in PCR and explain how PCR is used to amplify DNA. 2. Be able to list all important components used to set up a PCR reaction and understand each of their functions. 3. How is PCR an extension of DNA replication? Explain. 4. See also Lab 4 Alu Lecture 11 Protein Purification/Chromatography Reading: Ch. 9 Sec. 9.1-9.2; Sec. 9.3 pp. 252-253 Practice Problems: p. 9 Questions 2-4, 6-8 1. Why do we need to purify proteins? What characteristics of proteins are commonly used to purify them? 2. What is chromatography? How does column chromatography work? How do the two phases accomplish separation of proteins? 3. How do each of the following types of chromatography work: a. Affinity chromatography b. Gel filtration chromatography c. Ion exchange chromatography d. Hydrophobic interaction chromatography (See also Lab 3B/C) Lecture 12 Human Genome Project (Eric Lander lecture) 1. What is the Human Genome Project (HGP)? What were/are its goals? 2. What technical advances were necessary (e.g. automated sequencing) before the human genome could be sequenced? 3. What have we learned so far from the HGP about the human genome? E.g. how many genes do humans have vs. mice or Arabidopsis ? How are genes distributed in the genome? How much variation is there between individuals? 4. What is “junk DNA”? What might it be composed of? What is the purpose of
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Bio 18 Fall 2007 Midterm 2 Study Guide Page 2 these DNA sequences? 5. Why is comparison of the human genome sequence with those of other model organisms (e.g. mouse) critical to helping us to understand what genes, etc. are in our own genome? 6. What do we still need to figure out? Be able to list several examples of current/future challenges 7. What applications does knowledge from the HGP have? Be able to list several examples, from medicine, forensics, evolution, etc. 8. What ethical, legal, or societal considerations are there for use of information from the HGP? Be able to discuss some examples. Lecture 13
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bio18midterm2studyguidef07 - Bio 18 Fall 2007 Midterm 2...

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