Chlamy lab

Chlamy lab - Jennifer O'Brien Bio Lab : TA Joe 10/17/06...

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Jennifer O’Brien Bio Lab : TA Joe 10/17/06 Chalmy Lab Introduction: The objective of this lab is to understand the mating and inheritance patterns of mitochondria and chloroplasts in organisms. In this particular lab, we used a single celled organism; Chlamydomonas reinhardtii (Chlamy) to understand these concepts. Due to its unique life cycle, which is easy to handle and manipulate under laboratory conditions, we can mate different strands of chlamy to see and understand its modes of inheritance. Chlamy live in a vegetative state as haploids. They are either mt- or mt + (mating type + or - ) which are the two given “genders”. When the two mating types are mixed together in proper conditions (a deprivation of nitrogen), they will mate and form clumps of entangled flagellum. They then separate again and form a “quadrifladellate dikaryon”, which is essentially a cell with four flagella and two nuclei. Lastly, the two nuclei will combine together, and the cells will form a diploid zygote, which will then undergo meiosis to form four more vegetative haploid cell offspring. Chloroplasts are very sensitive to certain strains of antibiotics that kill bacteria. There are strong similarities between the “evolutionary origins” of chloroplast and bacteria and chloroplasts are very sensitive to certain strains of antibiotics that kill bacteria. In this lab, we used the two antibiotics streptomycin and erythromycin, to view mutant trains of chlamy that will be resistant to these antibiotics to view its mutant inheritance. We will analyze the mating of four different strains to view the inheritance of the chloroplast gene when Chlamy mating occurs:
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Mt +, str R, ery S X mt -, str S, ery R And Mt +, str S, ery R X mt -, str R, ery S CC-118 CC- 67 CC-333 CC- 119 The capital letter R, and S, means that the strain is either R-resistant or S- sensitive to the antibiotic specified. For the procedure we will be crossing the two crosses on two different complete
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Chlamy lab - Jennifer O'Brien Bio Lab : TA Joe 10/17/06...

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