Unformatted text preview: eaction:
CH4N2O + H2O → 2NH3 + CO2
The urease crystals contained only protein,
leading Sumner to propose that all enzymes
He received a Nobel Prize in 1946 for this
discovery. Discovery of enzymes
In the 1930s, Haldane postulated that weak
bonding interactions between an enzyme and
its substrate might be used to catalyze a reaction.
This key insight lies at the heart of our current
understanding of enzyme catalysis. Enzymes speed up the rates of
The active site of an enzyme is usually
a cleft or pocket where chemistry takes place.
A molecule that binds in the active site and
is acted upon by the enzyme is called
As simple equation for an enzyme-catalyzed
reaction can be written:
E + S ES EP E + P
where E is enzyme, S is substrate and P is product.
Enzymes do not alter the reaction equilibrium, but
they alter the forward and reverse reaction rates.
Enzymes remain unchanged after the reaction. Some examples...
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