microscope p 6-10

microscope p 6-10 - Stage: platform on which the specimen...

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Stage: platform on which the specimen slide is placed. It has a hole in its center permitting light to pass through. A mechanical movable stage fixture is affixed to the stage. It holds the specimen slide, permitting controlled movement of the specimen. Notice that “3” in the above figure is a moveable clip. It is necessary to open it to properly place a slide on the stage. Note the knobs next to the # 3. These control the mechanical stage. DO: Move the X-axis and Y-axis knobs. Specimen lighting may come from an external light source or a built-in light. If the light is external, then a mirror is used to ‘direct’ the light upward. If the light is built in, a condenser is used to focus the light into a beam. This scope has a built-in light. Locate the “barrel shaped” condenser. DO: Try moving condenser by pulling down on “barrel” or pushing up on “barrel”. Repeat movement while looking through the eyepieces. Leave the condenser in the ‘up’ position where the beam is tightly focused. Look at the top of the condenser. Attached to it is the diaphragm, a device used to control the width of the light beam, therefore, the amount of light passing upward. It is mechanical. Its opening is controlled a lever. DO: While looking at the top of the stage, move the diaphragm lever and note the diaphragm opening and closing. It functions much like the pupil of your eye. Repeat lever movement while looking through the eyepieces. Note the light ‘dims’.
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???????? What else performs a similar ‘dimming’ function? Locate the field iris diaphragm ring. DO: Twist it and note the iris action controlling the diameter of the light. Note also the iris glass surface. Dusty? While looking through the eyepieces, open the iris so that the ring of light fills the field of view. Coarse and fine focus knobs:
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This note was uploaded on 04/07/2008 for the course BIO 110 taught by Professor Phil during the Spring '08 term at South Carolina.

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microscope p 6-10 - Stage: platform on which the specimen...

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