BIOL 227 L2 F2020 Bacteria Viruses - Yazeed Ibsais (27033788).pdf

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Lab 2: Bacteria & Viruses - classify and count General Safety - worth reviewing in Covid 19 days If you are anaphylactic (hyper-allergenic) for bacterial spores, inform your instructor. Please be focused on your bench work, wandering and distraction increases the possibility of accidents, starting with contamination of your sources of information – this week cells, colonies and plaques. Exercise care when using open flame. Keep long hair and loose clothing away from the open flame. Do not place solvents such as alcohol near open flame. Extinguish the flame immediately after use. Always be aware of the need to use sterile techniques when manipulating microorganisms. This is necessary to prevent contamination in your experiments and spreading of microorganisms in the environment. Close the bacterial plates immediately after use. All contaminated materials/objects should be placed on the tray provided for this lab. Never place them on the lab bench or basket after use. Stains may permanently stain your clothes, especially crystal violet stain. Please work carefully . Report microbial spills to your instructor, for proper clean-up. Immediately rinse/wash your skin and clothing if it comes in contact with any spills. Keep fingers, pencils, bacteriological loops etc. out of your mouth. The slides that you have prepared must be discarded in the biohazard containers. Always clean up your bench area after you have finished. Disinfect the bench by swabbing down with 70% alcohol before leaving the lab. If you use an oil immersion lens, remove all traces of oil from the lens using lens paper moistened with 70% alcohol, before leaving the lab. Wash your hands before leaving the lab.
Introduction It is now certain that the best we can say about prokaryote diversity is it comprises 2 Domains and the diversity of each, Bacteria and Archa ea, is enormous. The most efficient way of estimating bacterial diversity is in an environmental sample, using rRNA sequence data to classify prokaryote diversity (see ) These tools are being used to detect Covid 19 viral loads in our wastewater at levels should allow us to detect and localize early-stage infections ( - test-waste-water-to-track-next-covid-19-wave/wcm/81042006-f27c-41fd-bbc6-08cb126ef4db ) Still, a gold-standard identification, for example one satisfying Koch’s postulates, requires that you can culture and characterize a species of interest. Developing a method of culturing bacteria is often a matter of experience, involving an understanding of its ecology, it’s probable metabolic requirements and limitations, culturing experience and often with some luck. Nonetheless, once you have a culture, then you can run more tests than for sequence, such as test its sequence-predicted metabolism . This is particularly important as horizontal transfer of genes for antibiotic resistance and other fitness -enhancing traits is becoming common . Regardless,

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