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BI-312: Recombinant DNATechnologyMarina Ioselevich, Ph.D.[email protected]Office Hours:Thursday 10:30 a.m. – 12:00 p.m. in Room 428
BI312 is an introduction toBiotechnologyBiology:thescience of lifeTechnology:Theapplication ofknowledge to meetthe goals, goods,and services desiredby people.any technique that uses living organisms or substances fromthose organisms, to make or modify a product, to improveplants or animals, or to develop microorganisms for specificuses- US Office of Technology Assessment
Biotechnology is everywhere!• agriculture• aquaculture• biomaterials• genomics• bioinformatics• horticulture• food/beverage• fermentation• therapeutics• vaccines• diagnostics• environment• veterinary• forestry• and lots more !Biotechnology at the Cutting Edge Video
Recombinant DNATechnologyDNARNAproteintraitRecombinant DNA technology: combination of two or moresequences that would not normally occur together
Biotechnology Resourcesex.phpIndustrial Biotechnology Associationof Canada (BIOTECanada) :Life Sciences Ontario (LSO):
BI-312: Recombinant DNATechnologyCourse Outline available via e-centennialLecture: 2h/weekLab: 2h/week§Restriction Enzymes - Agarose Gelelectrophoresis-DNA extraction§DNA cloning§Expression Vectors§Recombinant protein expression andpurification§SDS-PAGE§PCR/RT-PCR§DNA Sequencing & the Human GenomeProject§Bioinformatics• basic techniques inbiotechnology: DNA andprotein manipulation
BI-312: BOOKSRequired TextBiotechnology: An Introduction (2ndEdition), SusanBarnum, Thompson Brooks/Cole. ISBN-10:0534492967, ISBN-13: 9780534492960Lab ManualAvailable on e-centennial underʻLabʼ; PRINT, READ andbring to the lab. Some weeks may have pre-lab assignmentsposted as well.Additional ReadingBiotechnology: A laboratory Skills Course, StudentEdition, J. Kirk Brown, Bio-Rad Laboratories Inc.,2011. ISBN 978-0-9832396-0-4
BI-312: Recombinant DNATechnologyEvaluationLecture (60%)Lab (40%)• Lecture assignment (5%)• Midterm #1 (20% )• Lecture quiz (10%)• Final lecture test (25%)• reports, assignmentsand quizzes (35%)• Lab Performance (5%)* must pass both lecture AND lab in order to pass course•students who are absent from experimental procedures will notbe permitted to hand in the corresponding report•**a “C” grade is required for entrance into third year
Lecture InformationLecture notes will be posted on e-centennial prior to lecture each weekThese notes ARE NOT a substitute forbeing in class; exam material is based onwhat is DISCUSSED in class; lecture notesare a guide
Lab InformationnBE PREPAREDnPreparation is expected, and will beevaluatednPlease check instructions postednPrint and bring lab protocolnCome to lecture!!
Single cells (unicellular):bacteria and yeastMulticellular organisms (eg. Humans have anestimated 6 X 1013cells, of about 320 different types!!!)Amazing diversity!!!

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