mcb lab writeup 1 - Bacterial Growth Curve Report October...

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Bacterial Growth Curve Report October 5, 2007 TA: Santosh Pande Section #3369 On my honor, I have neither given nor received unauthorized aid in doing this assignment. X____________________________________________ Date_____________________
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Abstract Plotting each of their Optical Density readings at 20 minutes for a 100-minute span and plotting them against time on semi-log graph paper measured the generation time of E.Coli populations incubated in 2 tubes of Brain-Heart Infusion broth at 20°C and 37°C. This experiment was performed to see which temperature E.coli preferred by comparing generation times. After the points were plotted and generation times were extrapolated, it was found that the population incubated at 20°C had a generation time of 74 minutes while the population incubated at 37°C had a generation time of 48 minutes. This experiment determined that E.Coli thrives better closer to 37°C then to 20°C. Introduction This experiment deals with bacterial growth and different growth conditions to the find the ideal growth for the species of interest, Escherichia coli . Bacterial growth refers not to the growth of individual cells, but to the growth of number of cells in a certain population (Munro, 2007). The typical stages of bacterial growth can be represented by a generalized bacterial growth curve. This curve consists of four distinct stages. The first stage, the Lag phase, is when cells are transferred to a fresh medium and the cells need to adjust to the new conditions. Because of this adjustment period, no increase in cell number is observed. The second phase, or the Logarithmic stage, is when the cells have adapted to the new medium and begin to divide. This is the stage where generation time is measured. Once waste products build up and the environment of the medium changes, the number of cells dividing equals the number of cells dieing. This stage is known as the stationary phase. Finally, the death phase follows and this is the stage of no microbial growth. On the generalized bacterial growth curve with the number
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This note was uploaded on 04/07/2008 for the course MCB 3020L taught by Professor Ogden during the Fall '08 term at University of Florida.

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mcb lab writeup 1 - Bacterial Growth Curve Report October...

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