Chapter 13 Reading Guide.docx - CBEN 110 Reading Guide...

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CBEN 110Chapter 13Reading GuideComplete this reading guide as you read the textbook. You might not have to read every word onevery page, rather pay close attention to the questions in this guide and answer them as you workthrough the textbook. Also pay close attention to the terms that are underlined: these are keyterms that you should know the definitions of and be able to apply in new situations.Chapter 13 Learning Objectives:1.Describe the structure and function of DNA and RNA.2.Describe and interpret data from experiments that provided evidence that DNA is thehereditary material and that DNA replicates in a semiconservative manner.3.Explain the steps involved in DNA replication.4.Explain how errors in DNA replication are corrected.5.Explain how the different levels of chromatin structure influence cellular processes thatutilize DNA as a template.6.Describe and explain the basic techniques and tools of genetic engineering, specificallycloning, gel electrophoresis, PCR, DNA sequencing, and restriction digestionConcept 13.1Summarize what Griffith found about transformationof nonpathogenic bacteria into pathogenicbacteria. See Figure 13.3 for help.
Summarize what Hershey and Chase found about how bacteriophagesinfect bacteria, leadingthem to conclude that DNA, and not protein, was the genetic material of cells. See Figure 13.5 forhelp. Answer the “what if” question associated with Figure 13.5.
What did Chargaff discover about DNA diversity and the relative amounts of nucleotides in DNAstrands?
Using Figure 13.6 for help, draw a DNA nucleotide, labeling the phosphate, deoxyribose sugar,and a nitrogenous base. Which end of the nucleotide is the 5’ endand which is the 3’ end?
Read and analyze the “scientific skills exercise” on page 257. You will be answering the questionsassociated with this exercise as part of your pre-class assignment.

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