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Lab practice final

Lab practice final - 1 1 Review of project You began this...

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1. Review of project : You began this course by isolating genomic DNA from the organism __________. You cut the genomic DNA with the restriction enzyme SalI, which you also used to cut the plasmid, ______. You then mixed the genomic DNA fragments with the cut plasmid and ligated them together with the enzyme ___________. You then transformed the ___________ strain of E. coli cells with the recombinant plasmids, and plated the cells on agar plates containing ampicillin and X-gal. The _______ gene on the plasmid allowed cells with the plasmid to survive on the ampicillin plates, and the_____________ gene on the plasmid was used to differentiate cells with plasmids with inserts from those without inserts. If a white colony glowed, it meant you had successfully cloned the _____ operon into the plasmid presents in those cells. The size of the operon was ____ kb. In order to subclone the luxAB genes, you made overnights of the glowing colonies and prepared plasmids from them. You then used the enzymes _______ and _________ to cut the recombinant plasmid and ran the resulting fragments on and agarose gel.
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