Lab 10 - Bacterial Growth II

4 decline phase cells are now dying at an exponental

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Unformatted text preview: 7 1/ 24 0.008 ? 29 Lab 10. November 7- 9, 2012 Spectrophotometric Analysis EXAMPLE Tube Number Dilu?on Absorbance (OD600 nm) CFU/mL Undiluted — — 1.44 x 108 1 1/ 2 0.210 ? 2 1/ 4 0.117 ? 5 1/ 6 0.080 ? 3 1/ 8 0.061 ? 6 1/ 12 0.026 ? 4 1/ 16 0.020 ? 7 1/ 24 0.008 ? 30 Lab 10. November 7- 9, 2012 Spectrophotometric Analysis EXAMPLE Tube Number Dilu?on Absorbance (OD600 nm) CFU/mL Undiluted — — 1.44 x 108 1 1/ 2 0.210 7.2 x 107 2 1/ 4 0.117 3.6 x 107 5 1/ 6 0.080 2.4 x 107 3 1/ 8 0.061 1.8 x 107 6 1/ 12 0.026 1.2 x 107 4 1/ 16 0.020 9.0 x 106 7 1/ 24 0.008 6.0 x 106 Lab 10. November 7- 9, 2012 31 Combining your Serial DiluTons You can now construct a standard curve. •  Plot these points onto a piece of graph paper with absorbance (OD600nm) on the x- axis and concentraTon (CFU/mL) on the y- axis. •  Use this standard curve to convert between OD600 and CFU/mL for the bacterial growth curve in Experiment 20. 32 Lab 10. November 7- 9, 2012 Bacterial Standard Curve EXAMPLE Bacterial Concentra?on (CFU/mL) 140,000,000 120,000,000 100,000,000 80,000,000 60,000,000 40,000,000 20,000,000 0 0 0.05 0.1 0.15 0.2 0.25 Absorp?on (OD600) 0.3 0.35 0.4 Lab 10. November 7- 9, 2012 33 BACTERIAL GROWTH CURVE Modified from Experiment 20 Lab 10. November 7- 9, 2012 The Bacterial Growth Curve 1. Lag phase •  Cells are adjusTng to a new environment and preparing to divide. 2. Logarithmic (log) phase •  Cells begin replicaTng at an exponenTal rate. 3. StaTonary phase •  Due to t...
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This note was uploaded on 01/06/2013 for the course BIOL 1902 taught by Professor Murry during the Spring '13 term at University of Winnipeg.

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