Lab 10 - Bacterial Growth II

November 7 9 2012 methodology 12 13 lab 10 november

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Unformatted text preview: ecies. •  This curve is unique to the experimental condiTons used. If the experimental condiTons change (organism, media, atmosphere, etc), the curve is no longer valid. •  For most bacteria, moderate changes in temperature do not affect the standard curve. Lab 10. November 7- 9, 2012 HEALTH & SAFETY 10 11 Lab 10. November 7- 9, 2012 Health & Safety POTENTIAL PROBLEMS •  Infec?ous Agents SOLUTIONS •  First Aid Kit •  Personal Protec?ve Equipment •  Biological Waste Container Lab 10. November 7- 9, 2012 METHODOLOGY 12 13 Lab 10. November 7- 9, 2012 Chemotaxis Observe bacterial growth Serial Dilu?ons Count colonies on agar plates Construct a standard curve Bacterial Growth Curves Construct a bacterial growth curve Spectrophotometry Expt. 13: Media for the rouTne culTvaTon of bacteria Expt. 16: pH of the extracellular environment Lab 10. November 7- 9, 2012 CHEMOTAXIS 14 Lab 10. November 7- 9, 2012 Chemotaxis •  One plate per organism per condiTon (4 plates total) •  Organisms •  Escherichia coli •  Micrococcus luteus •  Agar Split Plates •  2 GYE/GYE (glucose yeast extract) •  2 GYE/H2O 15 16 Lab 10. November 7- 9, 2012 InoculaTons per organism (E. coli and M. luteus) x marks the locaTon of the inoculum GYE x GYE x x x x x x GYE x H2 O Lab 10. November 7- 9, 2012 SERIAL DILUTION Modified from Experiment 19 17 18 Lab 10. November 7- 9, 2012 +1mL +1mL +1mL +1mL +1mL +1mL undiluted broth +1mL 9mL H2O 9mL H2O 9mL H2O 9mL H2O 9mL H2O 9mL H2O 9mL H2O 100 10- 1 10- 2 10- 3 10- 4 10- 5 10- 6 10- 7 +1mL 10- 2 +1mL 10- 6 +1mL 10- 4 +1mL 10- 3 +1mL 10- 5 +1mL...
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This note was uploaded on 01/06/2013 for the course BIOL 1902 taught by Professor Murry during the Spring '13 term at University of Winnipeg.

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