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Spectrophotometric Determination of the Value of the Dissociation Constant of an Acid

Spectrophotometric Determination of the Value of the Dissociation Constant of an Acid

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S PECTROPHOTOMETRIC D ETERMINATION OF THE V ALUE OF THE D ISSOCIATION C ONSTANT OF AN A CID -B ASE I NDICATOR P URPOSE : T HE PURPOSE OF EXPERIMENT 16 IS TO USE A SPECTROPHOTOMETER TO DETERMINE THE CONCENTRATIONS OF BOTH ACIDIC AND CONJUGATE BASIC FORMS OF AN INDICATOR AND TO CALCULATE THE DISSOCIATION CONSTANT OF THE INDICATOR BASED ON THESE VALUES . P ROCEDURE : 1.) P IPET 1.0 M L OF INDICATOR STOCK SOLUTION INTO A 25 M L VOLUMETRIC FLASK . 2.) A DD TO THE FLASK A BUFFER SOLUTION MAKING THE TOTAL VOLUME EXACTLY 25 M L. 3.) S TOPPER THE FLASK AND MIX CONTENTS . 4.) L ABEL THE FLASK WITH P H OF SOLUTION . 5.) R EPEAT STEPS 1-4 FOR REMAINING BUFFER SOLUTIONS . 6.) U SE THE O CEAN O PTICS SPECTROPHOTOMETER TO DETERMINE THE ABSORBANCES AT DIFFERENT WAVELENGTHS . 7.) R INSE A CELL SEVERAL TIMES WITH FIRST SOLUTION , THEN FILL CELL LEAVING
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Unformatted text preview: HALF INCH AT THE TOP . D ETERMINE ABSORBANCE AT 430 NM AND AT 588 NM . R ECORD RESULTS ON TABLE . 8.) R EPEAT STEP 7 WITH ALL SOLUTIONS . 9.) S HUT DOWN PROGRAM AND COMPUTER . C HEMICALS : • B ROMOCRESOL P URPLE S OLUTION IN E THANOL • V ARIOUS B UFFER S OLUTIONS C HEMICAL R EACTIONS : • HI N + H 2 O H 3 O + + I N-E QUATIONS : • K A = + H3O I N-I N-• + = H K A HI N I N-• = A Ε C • = C A Ε • A CORR = A-A IMP • Ε 430 NM = A CORR I NDICATOR • HI N = A CORR Ε 430 • Ε 588 NM = A CORR I NDICATOR • I N-= A CORR Ε 588 •...
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