Spectrophotometric Determination of the Value of the Dissociation Constant of an Acid

Spectrophotometric Determination of the Value of the Dissociation Constant of an Acid

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S PECTROPHOTOMETRIC D ETERMINATION OF THE V ALUE OF THE D ISSOCIATION C ONSTANT OF AN A CID -B ASE I NDICATOR P URPOSE : T HE PURPOSE OF EXPERIMENT 16 IS TO USE A SPECTROPHOTOMETER TO DETERMINE THE CONCENTRATIONS OF BOTH ACIDIC AND CONJUGATE BASIC FORMS OF AN INDICATOR AND TO CALCULATE THE DISSOCIATION CONSTANT OF THE INDICATOR BASED ON THESE VALUES . P ROCEDURE : 1.) P IPET 1.0 M L OF INDICATOR STOCK SOLUTION INTO A 25 M L VOLUMETRIC FLASK . 2.) A DD TO THE FLASK A BUFFER SOLUTION MAKING THE TOTAL VOLUME EXACTLY 25 M L. 3.) S TOPPER THE FLASK AND MIX CONTENTS . 4.) L ABEL THE FLASK WITH P H OF SOLUTION . 5.) R EPEAT STEPS 1-4 FOR REMAINING BUFFER SOLUTIONS . 6.) U SE THE O CEAN O PTICS SPECTROPHOTOMETER TO DETERMINE THE ABSORBANCES AT DIFFERENT WAVELENGTHS . 7.) R INSE A CELL SEVERAL TIMES WITH FIRST SOLUTION , THEN FILL CELL LEAVING A
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Unformatted text preview: HALF INCH AT THE TOP . D ETERMINE ABSORBANCE AT 430 NM AND AT 588 NM . R ECORD RESULTS ON TABLE . 8.) R EPEAT STEP 7 WITH ALL SOLUTIONS . 9.) S HUT DOWN PROGRAM AND COMPUTER . C HEMICALS : B ROMOCRESOL P URPLE S OLUTION IN E THANOL V ARIOUS B UFFER S OLUTIONS C HEMICAL R EACTIONS : HI N + H 2 O H 3 O + + I N-E QUATIONS : K A = + H3O I N-I N- + = H K A HI N I N- = A C = C A A CORR = A-A IMP 430 NM = A CORR I NDICATOR HI N = A CORR 430 588 NM = A CORR I NDICATOR I N-= A CORR 588...
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This lab report was uploaded on 04/07/2008 for the course CHEM 1114 taught by Professor Prof.adams during the Spring '08 term at SMU.

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Spectrophotometric Determination of the Value of the Dissociation Constant of an Acid

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