The Study of Cellular Respiration Under Different Conditions
16 November 2010
All organisms go through the process of cellular respiration.
This vital process helps
the cells get the energy they need to survive.
Energy is needed in all organisms for life’s daily
Using different amounts of succinate, we were able to examine the affects it had on
cellular respiration with the help of DPIP dye.
In cellular respiration succinate oxidized and
gives its electrons to fumerate, by having the blue DPIP as an artificial electron acceptor, it
intercepts fumerate and becomes reduced.
The blue color gradually turns colorless as DPIP
gets reduced in the reaction.
It was hypothesized that an increase of succinate would result in
an increase rate of cellular respiration.
The data collected in this experiment helped support
this hypothesis in showing that the tube that obtained the greatest amount of succinate
resulted in a quicker change of blue to colorless compared to the tubes containing a lesser
amount of succinate.
When more succinate is present it is able to give off more electrons and
DPIP is able to accept more electrons in the oxidative-reduction reaction.
In this experiment we investigated cellular respiration in an isolated mitochondria and
studied the rate of cellular respiration under different conditions.
It is important for students
to know how cells get their energy through catabolic processes and how the rate of cellular
respiration is affected certain substances.
Cellular respiration is a process that breaks down
organic molecules and yields energy in the form of ATP (adenosine triphosphate).
two types of cellular respiration, aerobic respiration is when oxygen is present to be consumed
by the reaction and anaerobic respiration is when no oxygen is present.
For this experiment
we focused on aerobic respiration. This process consists of three main stages; glycolysis
which is the breakdown of sugar, Krebs cycle which is the transfer energy to the electron
transport chain, and oxidative phosphorylation which is the generation of water and ATP.
In the Krebs cycle the enzyme succinate dehydrogenase converts
succinate to fumerate in a oxidative-reductive reduction reaction.
An enzyme is a protein that
serves as a catalyst for a reaction.
It speeds up a chemical reaction by lowering the activation
Enzymes can be influenced by different levels of pH, temperature, and chemicals
which can alter the rate of a reaction.
An oxidation reaction is a reaction in which a substance
loses electrons, this substance is called the reducing agent because it donates an electron.
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