The Study of Cellular Respiration Under Different Conditions Biology 107 Section 5 16 November 2010
Abstract All organisms go through the process of cellular respiration. This vital process helps the cells get the energy they need to survive. Energy is needed in all organisms for life’s daily activities. Using different amounts of succinate, we were able to examine the affects it had on cellular respiration with the help of DPIP dye. In cellular respiration succinate oxidized and gives its electrons to fumerate, by having the blue DPIP as an artificial electron acceptor, it intercepts fumerate and becomes reduced. The blue color gradually turns colorless as DPIP gets reduced in the reaction. It was hypothesized that an increase of succinate would result in an increase rate of cellular respiration. The data collected in this experiment helped support this hypothesis in showing that the tube that obtained the greatest amount of succinate resulted in a quicker change of blue to colorless compared to the tubes containing a lesser amount of succinate. When more succinate is present it is able to give off more electrons and DPIP is able to accept more electrons in the oxidative-reduction reaction. Introduction In this experiment we investigated cellular respiration in an isolated mitochondria and studied the rate of cellular respiration under different conditions. It is important for students to know how cells get their energy through catabolic processes and how the rate of cellular respiration is affected certain substances. Cellular respiration is a process that breaks down organic molecules and yields energy in the form of ATP (adenosine triphosphate). There are two types of cellular respiration, aerobic respiration is when oxygen is present to be consumed by the reaction and anaerobic respiration is when no oxygen is present. For this experiment we focused on aerobic respiration. This process consists of three main stages; glycolysis which is the breakdown of sugar, Krebs cycle which is the transfer energy to the electron
transport chain, and oxidative phosphorylation which is the generation of water and ATP. (Campbell 162-177) In the Krebs cycle the enzyme succinate dehydrogenase converts succinate to fumerate in a oxidative-reductive reduction reaction. An enzyme is a protein that serves as a catalyst for a reaction. It speeds up a chemical reaction by lowering the activation of energy. Enzymes can be influenced by different levels of pH, temperature, and chemicals which can alter the rate of a reaction. An oxidation reaction is a reaction in which a substance loses electrons, this substance is called the reducing agent because it donates an electron. A
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