Determination of Fe by UV Spectrometry - Brooks Micheal...

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Brooks Micheal Brooks Quantitative Analysis Wei 9 November 2010 Determination of Fe by UV-Vis Spectrometry Introduction: A complex of iron (II) is formed with 1,10-phenanthroline, Fe(C 12 H 8 N 2 ) 3 2+ , and the absorbance of this colored solution is measured with a spectrophotometer. The spectrum is plotted to determine the absorption maximum. Hydroxylamine (as hydrochloride salt to increase solubility) is added to reduce Fe 3+ to Fe 2+ and to maintain it in that state. 4 Fe 3+ + 2NH 2 OH → 4Fe 2+ + N 2 O + 4H + + H 2 O Materials: Phenanthroline Water Ferrous Ammonia Sulfate Iron (II) Sulfuric Acid Hydroxylammonium Chloride Sodium Acetate Unknown Volumetric Flasks Procedure: 1.) Pipet 1.00, 2.00, 5.00, 10.00, and 25.00mL of iron solution into 100mL volumetric flasks.
Brooks 2.) Add 50mL of distilled water into a 100mL volumetric flask. 3.) Add the unknown to another volumetric flask. 4.) Add 1.0mL Hydroxylammonium chloride and 5.0mL phenanthroline to the volumetric

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