Lecture 10 (deleted 4f50fba2-1dcdc3-ad76d0c5)

6 the recombina7on frequency between ss and e is 43

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Unformatted text preview: s r c+ Which is the middle locus? (A) s, (B) r, (C) c, (D) Cannot be determined, (E) I am not sure. EXPLANATION: The non ­recombinant progeny differ from the double ­crossover progeny at the c locus, which suggests the c locus is in the middle of the other two loci. 9 Step 2: Calculate recombina7on frequencies. As before, we add up the recombinant progeny, dividing this number by the total number of progeny, and then mul7ply by 100%. Example: The recombina7on frequency between st and ss is: (50 + 52 + 5 + 3)/755 x100% = 14.6% The recombina7on frequency between ss and e is: (43 + 41 + 5 + 3)/755 x100% = 12.2% Note: red dash indicates posi7on of crossover 10 5 2/23/12 Let’s build a gene7c map: three ­point testcross Step 1: Determine the order of the 3 genes. The ss locus is in the middle posi7on. 26.8 m.u. Step 2: Calculate recombina7on frequencies. The recombina7on frequency b...
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This note was uploaded on 01/30/2013 for the course BIO LS1B taught by Professor Andrewberry,hopihoekstra,maryellenruvolo during the Spring '12 term at Harvard.

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