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Unformatted text preview: s cut DNA and
generate blunt ends or sticky ends? Do you know the principle of the formation of recombinant
What are the molecules that are used in the generation of
What are the enzymes that are necessary for the cleavage of
chromosomal DNA and plasmid DNA?
How can DNA fragments be attached to a plasmid vector?
Can you explain what is PCR (polymerase chain reaction)?
Do you need any special equipment for PCR?
Do you need to use specific primers to amplify a gene of
your interest? Do you need a DNA template in PCR?
• Do you need to use a special heat-resistant DNA polymerase
in PCR? What is the name of the bacteria from which DNA
polymerase is isolated for use in PCR?
What are the steps in PCR, What are temperatures used for
denaturation of DNA, annealing of the primers to the DNA
template, and extension of the polymerizing DNA chain?
What is the name of the scientist, who won a Nobel Prize for
inventing the PCR method?
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