ch7brq - Chapter 7B Genomes and Chromosomes Figure 7-19...

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Chapter 7B Genomes and Chromosomes
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Figure 7-19 RNase H removing the RNA primer .
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http://www.youtube.com/watch?v=Luw5_z8mIrI&feature=related DNA replication
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Figure 7-20 Location of replicative DNA polymerase in living cells . Bacillus subtilis cells containing PolC-GFP (DNA polymerase III tagged with green fluorescent protein) A. PolC localized as discrete green foci B. DNA was stained with DAPI (blue fluorescence)
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Figure 7-21 Terminating replication of the chromosome Holliday Junction
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Holliday junction http://www.youtube.com/watch?v=gQFKdA3VgEg&NR=1 http://www.youtube.com/watch?v=SROcb5h1--w&NR=1 http://biology-animations.blogspot.com/2007/12/holliday-junction- http://biology-animations.blogspot.com/2007/12/holliday-junction- http://www.youtube.com/watch?v=gQFKdA3VgEg&NR=1
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Plasmids q Plasmids are extra-chromosomal DNA q Plasmids replicate autonomously q Episome designates a plasmid that can integrate into the bacterial genome q Plasmids can be used as a cloning vector q Plasmids can be cured (eliminated) spontaneously or induced by certain chemicals, such as acridine dyes which insert into DNA q Plasmids can replicate in two different ways 1. Bidirectional replication and/or by 2. Rolling-circle replication
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Figure 7-23 Plasmid map.
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Figure 7-24 Plasmid replication: rolling- circle model .
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Figure 7-25 Molecular model of plasmid segregation . Note the involvement of an actin-like filament, which is mediated by ParM, and the requirement for energy
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Plasmids are transferred between cells q Plasmids play a role in the evolution of bacteria, and in the acquisition of virulence factors (ex. Bacteriocins), antibiotic resistance genes, q Plasmids can be transferred by q Conjugation This process mediated by sex pili q Transformation This process occurs in competent bacteria (bacteria with the ability to uptake DNA). Competence occurs naturally or can be induced chemically or physically q Transduction This process is mediated by bacteriophages
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q Conjugation Some plasmids (conjugative plasmids) can be transferred by conjugation (contact between 2 cells). Some non transferrable plasmids can be transferred to another cell, if the donor cell has a conjugative plasmid q
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ch7brq - Chapter 7B Genomes and Chromosomes Figure 7-19...

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