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Unformatted text preview: s/an Figure 7-30 Dideoxynucleotide. Figure 7-31 DNA sequencing using fluorescently tagged
dideoxynucleotides to randomly stop chain elongation. Figure 7-32 Rapid DNA sequencing using an automated DNA sequencer. Metagenomics
Use of modern genomic techniques to study microbial
Basis: each species is determined by the sequence of the
16S small ribosomal RNA (rRNA)
q Welcome to the “OMICS” Era
Genomics, Transcriptomics, Proteomics, Pharmacogenomis,
Metabolomics, Metagenomics, etc……. Review questions
Can you name the enzyme that mediates the removal of
RNA primers in DNA replication?
What is the function of RNA primers in replication?
How can microbiologists locate DNA polymerase in
living bacterial cells?
Can DNA be stained with a fluorescent molecule? Can you
name the blue fluorescent dye that is used to stain DNA?
Where does replication of genomic DNA in E. coli end? Are
the two copies readily found separated or are they
attached at the terminal site of replication?...
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