Dnalcorgresourcesan figure 7 30 dideoxynucleotide

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Unformatted text preview: s/an Figure 7-30 Dideoxynucleotide. Figure 7-31 DNA sequencing using fluorescently tagged dideoxynucleotides to randomly stop chain elongation. Figure 7-32 Rapid DNA sequencing using an automated DNA sequencer. Metagenomics Use of modern genomic techniques to study microbial communities q Basis: each species is determined by the sequence of the 16S small ribosomal RNA (rRNA) q Welcome to the “OMICS” Era Genomics, Transcriptomics, Proteomics, Pharmacogenomis, Metabolomics, Metagenomics, etc……. Review questions Can you name the enzyme that mediates the removal of RNA primers in DNA replication? • What is the function of RNA primers in replication? • How can microbiologists locate DNA polymerase in living bacterial cells? • Can DNA be stained with a fluorescent molecule? Can you name the blue fluorescent dye that is used to stain DNA? • Where does replication of genomic DNA in E. coli end? Are the two copies readily found separated or are they attached at the terminal site of replication?...
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This note was uploaded on 01/25/2013 for the course MCB 3020 taught by Professor Mydao during the Spring '11 term at University of South Florida.

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