Mcgraw hillcomsites gene cloning with in fusiontm in

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Unformatted text preview: Gene cloning with In-fusionTm (In Vitrogen) Conjugation Plasmid transfer by conjugation Conjugation: Transfer of the F plasmid Conjugation: Transfer of chromosomal DNA Transduction Generalized Transduction Specialized Transduction Generalized transduction Specialized transduction by temperate phage DNA in prokaryotes versus eukaryotes Bacteria: Continuous coding sequence Eukaryotes: Discontinuous coding sequences Figure 7-27 Bacterial DNA restriction endonucleases Figure 7-28 Formation of recombinant DNA molecules. PCR Polymerase Chain Reaction Kary B. Mullis The Nobel Prize in Chemistry 1993 Kary B. Mullis, Michael Smith Figure 7-29 The polymerase chain reaction. Steps in PCR Cloning of PCR product DNA sequencing
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