Lecture 8 Preliminary upload

Lecture 8 Preliminary upload - Things you should have...

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Unformatted text preview: Things you should have learned from 2/21 lecture DNA replication is semiconservative, i.e. each new DNA molecule consists of one old and one new strand DNA molecules grow at the 3 end DNA polymerases can not initiate a new chain, only elongate an RNA or DNA strand DNA replication is primed by RNA molecules synthesized by the primase (or primasome in eukaryotes) Leading strand replication is made in long segments Lagging strand replication is made in a larger number of smaller segments DNA segments are sealed together by ligase after removal of RNA primer DNA is unwound by helicase (topoisomerase) at front of replication fork Most replication errors are corrected during or after replication Nucleotide nomenclature dATP deoxy adenosine ribonucleotide ATP adenosine ribonucleotide dNTP Mix of the four deoxy ribonucleotides NTP Mix of the four ribonucleotides Determining the sequence of DNA Thousands of DNA pieces Purify DNA Vector Cut with restriction enzyme Ligate Molecular cloning of random pieces of DNA Transform DNA into bacteria http://nition.com/en/products/pm-1_s.htm Automation is essential DNA from each clone clone is prepared The sequence of the DNA insert in each clone is determined O CH 2 O Base (A, T, G, C) OH P P P P = phosphate Deoxyribonucleoside triphosphate 1 2 3 4 5 O...
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Lecture 8 Preliminary upload - Things you should have...

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