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Unformatted text preview: ormal‐phase packing, the stationary phase is polar and the mobile phase is relatively non‐polar. (e) In ion‐pair chromatography a large organic counter‐ion is added to the mobile phase as an ion‐pairing reagent. Separation is achieved either through partitioning of the neutral ion‐pair or as a result of electrostatic interactions between the ions in solution and charges on the stationary phase resulting from adsorption of the organic counter‐ion. 7. (12 points, Modified from Skoog 28‐20) Two components in an HPLC separation have retention times that differ by 15 seconds. The first peak elutes in 9.0 minutes and the peak widths are approximately equal. The dead time tM was 65 seconds. (With or without using a spreadsheet—your choice) Find the minimal number of theoretical plates needed to achieve the following resolution Rs values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25, 1.50, 1.75, 2.0, 2.5. (Note: In this case, do not worry about significan...
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This note was uploaded on 02/05/2013 for the course CHEM 420 taught by Professor Kelleher during the Spring '08 term at University of Illinois, Urbana Champaign.
- Spring '08