1 a 54 b 133 c 141 d 216 widthofpeakbasew minutes 041

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Unformatted text preview: 164 mL A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention time, minutes Nonretained 3.1 A 5.4 B 13.3 C 14.1 D 21.6 Width of Peak Base (W), minutes — 0.41 1.07 1.16 1.72 Calculate: a) the number of plates from each peak. b) the mean and the standard deviation for N. c) the plate height for the column. (a) 2 points for setup, 1 point for each answer, ‐0.5 for sig figs ⎛t ⎞ N = 16 ⎜ R ⎟ ⎝W ⎠ 2 2 ⎛ 13.3 ⎞ 3 for B: N = 16 ⎜ ⎟ = 2472 or 2.47x10 or 2470 plates ⎝ 1.07 ⎠ 2 ⎛ 21.6 ⎞ for D: N = 16 ⎜ = 2523 or 2.52x103 or 2520 plates 1.72 ⎟ ⎝ ⎠ ⎛ 5.4 ⎞ 3 for A: N = 16 ⎜ ⎟ = 2775 or 2.8x10 or 2800 plates ⎝ 0.41 ⎠ ⎛ 14.1 ⎞ = 2365 or 2.36x103 or 2360 plates for C: N = 16 ⎜ 1.16 ⎟ ⎝ ⎠ 2 2 (b) 1 point for...
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