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Unformatted text preview: Garrett Fedderson Tyler Fick Avi Kolel Emmanuel Koli DNA Sugar Phosphate Base Group What can DNA do? A, C, T or G These bases interact with each other to form the "twisted ladder" shape of DNA Make proteins Express/Suppress genes Generally decide WHO WE ARE Ex. PAX6 in Fruit Flies Corals DNA is not only in humans Corals have DNA, similar to human DNA
Same "twistedladder" structure Ribosomal DNA Codes for ribosomes in the cell Ribosomes translate DNA to make proteins for different functions Certain portions of rDNA are highly conserved Primers can be made to complementary copy on the DNA strand Ex. 18S & 28S primer sites PCR Use certain primers to isolate portions of the rDNA to make a specific segment Then, we can compare the length of the segment to different species A large difference in the length of segment between two species could indicate different species Sequencing would give a more detailed look at the fragment allowing for better comparison PCR Results Once PCR is run, our samples are put through a gel.
This gel will give us the lengths of our fragments We expected to find 823824 bp fragments on our gel. This indicates that coral rDNA was isolated. ...
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- Spring '08