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Unformatted text preview: in zebrafish embryos in vivo? (Be sure to describe the basic steps in the
experiment and the predicted results) (3 marks)
er ID: 4251 r ID : dd 42 Down y. 51 Downloader ID: 4251 Test ID:
t ID: 568 lo ad
e bu Engineer separate enhancer-reporter constructs using upstream regulatory regions
from gene VT-A (0.5 marks) and gene VT-B (0.5 marks) as well as a reporter (e.g.
EGFP) (0.5 marks)
Generate trangenic zebrafish embryos for each construct (0.5 mark)
Show that each enhancer can direct the expression of reporter to either brain (VT-A) or
heart (VT-B) (0.5 marks each)
ader ID: 568
Test ID: : tu de Downlo 8 nt - 56 - wn ID 51 st 42 : ID Te st
ID : 56 8 Engineer enhancer-reporter construct (0.5 marks) using human VT gene (0.5 marks)
upstream regulatory region and reporter (0.5 marks)
Generate zebrafish transgenics (0.5 marks)
Expect to see reporter expression in brain AND heart (1 mark)
r ID: ade de nl
oa 1 425 Do
w Dow - Te on to .s (c) Using zebrafish transgenic technology, describe how you would prove that the expression...
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- Fall '12