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functional enzyme (1 mark)
Transposase makes blunt-ended cuts in donor DNA (which releases the transposon
DNA) and staggered (5’ overhang) cuts in target DNA (1 mark)
Transposase ligates the transposon DNA to the 5’ single stranded ends of the target
DNA (1 mark)
Cellular DNA Pol extends 3’ cut ends and cellular ligase joins 3’ ends to transposon 5’
ends (generating direct repeats on either side of newly integrated transposon) (1 mark)
568 t ID: Tes 4251
ader ID: Downlo Oxdia @ http://www.oxdia.com
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8 Tes (c) What experiment would you perform to prove that RNA Pol II has “escaped” the promoter of a
given gene and is undergoing productive transcription elongation within cells? Be sure to state
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This note was uploaded on 02/08/2013 for the course CSB 349 taught by Professor Moses during the Fall '12 term at University of Toronto- Toronto.
- Fall '12