The test total id wn 4 briefly describe the 4 main

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Unformatted text preview: ed into a functional enzyme (1 mark) Transposase makes blunt-ended cuts in donor DNA (which releases the transposon DNA) and staggered (5’ overhang) cuts in target DNA (1 mark) Transposase ligates the transposon DNA to the 5’ single stranded ends of the target DNA (1 mark) Cellular DNA Pol extends 3’ cut ends and cellular ligase joins 3’ ends to transposon 5’ ends (generating direct repeats on either side of newly integrated transposon) (1 mark) 568 t ID: Tes 4251 ader ID: Downlo Oxdia @ http://www.oxdia.com This test is copyrighted by the uploader and/or course instructor. Downloader id is shown and also encrypted throughout the document. Unauthorized reproduction/distribution is strictly prohibited. Solution (if any) is NOT audited, so use at your discretion. Te st ID : 56 8 Tes (c) What experiment would you perform to prove that RNA Pol II has “escaped” the promoter of a given gene and is undergoing productive transcription elongation within cells? Be sure to state why you...
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This note was uploaded on 02/08/2013 for the course CSB 349 taught by Professor Moses during the Fall '12 term at University of Toronto- Toronto.

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