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Unformatted text preview: For instance, there are large differences in calcium permeabilities depending on the neuronal nAChRs subunit constitution. These channels are also selective for cations and must be expressed with both and subunits to be functional. By applying different chemicals you can elucidate which type of agonists/antagonists bind the receptor. Using inside-out/outside-out patches, you could determine stoichiometric relationships of ion flux, and permeability. Using a voltage-clamp you could determine any sort of voltage-dependence. Member of the cys-loop family? Comparison of our derived cDNA sequence to that of genetic databases will reveal similarities between out receptor of interest and know cys-loop family receptors. The M2 domain is evolutionarily conserved in glycine, GABA A, and nAChRs, and would presumably show up in a novel receptor as well....
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This essay was uploaded on 04/07/2008 for the course NSCI 5461 taught by Professor Newman during the Spring '08 term at Minnesota.
- Spring '08