Unformatted text preview: For instance, there are large differences in calcium permeabilities depending on the neuronal nAChRs subunit constitution. These channels are also selective for cations and must be expressed with both α and β subunits to be functional. By applying different chemicals you can elucidate which type of agonists/antagonists bind the receptor. Using inside-out/outside-out patches, you could determine stoichiometric relationships of ion flux, and permeability. Using a voltage-clamp you could determine any sort of voltage-dependence. Member of the cys-loop family? Comparison of our derived cDNA sequence to that of genetic databases will reveal similarities between out receptor of interest and know cys-loop family receptors. The M2 domain is evolutionarily conserved in glycine, GABA A, and nAChRs, and would presumably show up in a novel receptor as well....
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- Spring '08
- DNA, complimentary mRNA sequence, nAChRs, mRNA/amino acid sequence