Lab Report 1 Enzymes

Lab Report 1 Enzymes - Enzyme Lab Report Introduction...

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Enzyme Lab Report Introduction Enzymes are irrefutably crucial in the regulation of human metabolic reactions because they allow for life processes to keep pace with demand. By binding to the reactant molecule, or substrate, in an induced fit model, enzymes effectively lower the activation barrier and thereby catalyze the reaction. However, enzymes only function when conditions such as temperature and pH level fall within the optimal range or they run the risk of denatured proteins, rendering the enzyme ineffective. The purpose of this investigation is to better understand the correlation between factors such as pH and temperature, and enzyme activity. Determining optimal conditions and observing the effects on enzyme activity created by operating outside of this range will serve to demonstrate what changes the human body faces under less than desirable conditions. By observing the different rates of reaction for the LDH-catalyzed conversion of pyruvate to lactate via the amount of NADH present, we can clearly see how temperature and pH levels indirectly affect reactions by altering enzyme activity. Methods
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This lab report was uploaded on 04/07/2008 for the course APK 2105 taught by Professor Brooks during the Spring '07 term at University of Florida.

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Lab Report 1 Enzymes - Enzyme Lab Report Introduction...

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