BiochemLabF2012.SecondExamReviewQuestions

9describeanddistinguishbetweenthesethreetypesofchromat

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Unformatted text preview: AGE;
also
indicate
why
proteins
 move
from
the
gel
to
the
nitrocellulose
membrane
in
our
blotting
procedure.
 
 
 6.
Define
what
a
monoclonal
antibody
is
and
what
determines
it
specificity.
 
 
 7.

Describe
why
the
same
secondary
antibody
can
be
used
in
a
variety
of
different
of
 Western
blotting
experiments
(i.e.
in
experiments
to
detect
different
proteins.)
 
 
 8.
Name
the
two
types
of
cells
that
are
used
in
making
hybridomas
for
monoclonal
 antibody
formation
and
indicate
the
contributions
of
each
type
of
cell
in
the
 hybridomas...
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